Development of a rapid malaria LAMP-MS assay for diagnosis of malaria infections

Development of a rapid malaria LAMP-MS assay for diagnosis of malaria infections

Blog Article

Abstract Malaria remains a critical global health concern, here especially in tropical and subtropical regions, where it causes substantial morbidity and mortality.Current diagnostic methods, such as microscopy and PCR-based assays, are reliable but often impractical in resource-limited settings due to their dependency on complex equipment and skilled personnel.This study developed a novel malaria diagnostic platform by combining the Chelex-100/boiling DNA extraction method with a Loop-mediated Isothermal Amplification-MicroScanner (LAMP-MS) assay.

The Chelex-100/boiling method is simpler and more cost-effective than conventional DNA extraction processes, making it suitable for use in resource-limited settings.The LAMP-MS assay enables multiplex detection through a microchip design with four chambers.Each chamber of the microchip is preloaded with specific primers targeting Pan, Plasmodium falciparum (Pf), Plasmodium vivax (Pv), and an internal control, minimizing non-specific amplification in multiplex LAMP reactions.

In a clinical evaluation of 260 samples, the assay demonstrated a sensitivity of 97.5% for the Pan target and 100% for the Pf-specific target in the 80 Plasmodium falciparum (Pf) clinical samples.Similarly, for the 80 Plasmodium vivax (Pv) clinical samples, the assay achieved a sensitivity of 95% for the canon imageclass mf227dw Pan target and 94% for the Pv-specific target.

Notably, in the 100 non-infected clinical samples, the assay exhibited 100% specificity, with no false positives observed.These findings suggest that LAMP-MS is a rapid and reliable alternative to PCR-based methods, especially in resource-limited environments.